Table 1 Overview of crosses to determine the effect of age on horizontal transmission of Microsporidia MB in An. arabiensis mosquitoes. Microsporidia MB-negative insectary mosquitoes (recipients) were crossed with Microsporidia MB-positive F1 mosquitoes (mates)
Age group | Male | Female | ||
|---|---|---|---|---|
Insectary/F1 | Microsporidia MB | Insectary/F1 | Microsporidia MB | |
Control crosses | ||||
 3–4 days | Insectary | Uninfected | Insectary | Uninfected |
 7–8 days | Insectary | Uninfected | Insectary | Uninfected |
 10–11 days | Insectary | Uninfected | Insectary | Uninfected |
Experimental crosses | ||||
Male-to-female transmission | ||||
 3–4 days | F1 | Infected | Insectary | Uninfected |
 7–8 days | F1 | Infected | Insectary | Uninfected |
 10–11 days | F1 | Infected | Insectary | Uninfected |
Female-to-male transmission | ||||
 3–4 days | Insectary | Uninfected | F1 | Infected |
 7–8 days | Insectary | Uninfected | F1 | Infected |
 10–11 days | Insectary | Uninfected | F1 | Infected |