Table 1 Information on the eight primers designed for the dual multiplex PCR assay (PCR1, PCR2) to identify the six species within the Barbirostris Complex

fBDSVW

Common to 5 species

CGGATCGCATTATGTTGAAGG

1, 2

fBar

An. barbirostris

CTGTTACACACGGTCCAAAAG

208

1

fCamp

An. campestris

GTTAGAAAATGGCAACATGAGCAA

1

rBar&Van

An. barbirostris;

An. vanderwulpi

ATGCTTAAATTTAGGGGGTAGTC

388;

401

1

rVan&Dis

An. vanderwulpi;

An. dissidens

CCCGAAAAAGAAGATGGTGAACA

141;

141

1

rCamp

An. campestris

CTCCACAAATTTCAGAACATTGTCC

612

1

rSaeu2

An. saeungae

TTCGCAAACCTATCGACTCC

378

2

rWej

An. wejchoochotei

GGGTGTGTGCTGGAGAAA

245

2