Screening the Global Health Priority Box against Plasmodium berghei liver stage parasites using an inexpensive luciferase detection protocol

Table 1 Preparation of FLAR buffer

Reagent	[Stock]	[Working]	For 20ml FLAR
Tricine	200 mM	20 mM	2 ml
MgSO4	50 mM	2.67 mM	1.068 ml
MgCO3	10 mM	1.07 mM	2.14 ml
EDTA	10 mM	100 µM	200 µl
DTT	500 mM	20 mM	800 µl
d-luciferin	25 mM	100 µM	80 µl
ATP	25 mM	125 µM	100 µl
Cell Culture Water			13.6 ml

Buffer is made immediately before the assay endpoint. A 20 mL volume is sufficient for addition of up to 200 µL per well of a 96-well plate, 50 µL per well of a 384-well plate, or 20 µL per well of a 1536-well plate (with a margin remaining)

ISSN: 1475-2875