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Fig. 4 | Malaria Journal

Fig. 4

From: Correlative light-electron microscopy methods to characterize the ultrastructural features of the replicative and dormant liver stages of Plasmodium parasites

Fig. 4

Imaging of P. cynomolgi hypnozoites using IFA-CLEM. A Micrographs showing a P. cynomolgi hypnozoite at 7 dpi in a primary NHP hepatocyte stained for UIS4 (green) and nucleic acids (Hoechst, blue) and imaged with fluorescence and phase contrast microscopy. Scale bar is 20 μm. The white arrows on the zoom-in micrograph (top right of overlay) show two DNA punctae in the hypnozoite. The same hypnozoite is also represented on a TEM micrograph obtained using IFA-CLEM. Scale bar is 1 μm. B–E Low-magnification TEM micrographs of additional hypnozoites at 7 dpi. Note the host mitochondria (MH) positioned in proximity to the PVM of hypnozoites. NPc, P. cynomolgi nuclei; Api, apicoplast. Scale bars are 1 μm

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Malaria Journal

ISSN: 1475-2875

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