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Fig. 2 | Malaria Journal

Fig. 2

From: Correlative light-electron microscopy methods to characterize the ultrastructural features of the replicative and dormant liver stages of Plasmodium parasites

Fig. 2

Imaging of P. berghei liver stages using IFA-CLEM. A Diagram outlining the IFA protocol used to perform IFA-CLEM. A representative image of a 2-day old P. berghei liver stage in Huh7 cells stained for the PVM protein UIS4 (red) and Hoechst (blue) is shown. Scale bar is 20 μm. Low-magnification TEM micrographs for liver stages imaged with GFP-CLEM (B) and IFA-CLEM (C). Scale bars are 5 μm (B-i, B-ii, C-i and C-ii) or 10 μm (B-iii and C-iii). D Higher-magnification GFP-CLEM (top row) and IFA-CLEM (bottom row) micrographs showing the parasitophorous vacuole (PV) space, P. berghei (Pb) nuclei, the P. berghei mitochondrial network, apicoplasts, P. berghei vacuoles and the P. berghei endoplasmic reticulum (ER). NH, host nucleus; PVM, parasitophorous vacuole membrane; PPM, parasite plasma membrane. Scale bars are 500 nm

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Malaria Journal

ISSN: 1475-2875

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