Fig. 5

Inhibitory effects of anthracyclines on P. falciparum 5′–3′ DNA helicase. Lane 1 is the heat-denatured substrate. Lane 2 is negative control and lanes 3 and 20 are positive controls. Lanes 4–7 are nogalamycin at concentrations of 10, 5, 1, 0.1 μM, respectively. Lanes 8–19 are aclarubicin, daunorubicin, and doxorubicin at the same series of drug concentrations at 10, 1, 0.1, 0.01 μM, respectively. Lane 21 is an enzyme with 0.2% (v/v) DMSO