Fig. 4

Unwinding activity of P. falciparum 5′ to 3′ DNA helicase with different substrates. A the M13-17-mer partial duplex. B the 41-mer blunt end. C the M13-44-mer with 3′ hanging tail. D the M13-45-mer with 5' hanging tail. E the 5′ to 3′ hanging tails on the same M13 template. Asterisks denote the 32P-labelled end. Lane 1 is the control without enzyme, Lane 2 is a reaction with partially purified enzyme, and Lane 3 is the heat-denatured substrate. UD: unwound DNA