Fig. 3

Directionality of P. falciparum 5′ to 3′ DNA helicase activity. The 43-mer linear partial duplex was used as a substrate and then digested with SmaI. Lane 1 corresponds to a heat-denatured substrate, lane 2 is negative control (reaction without enzyme), and lane 3 is a reaction with 0.2 mg of partially purified P. falciparum 5′–3′ DNA helicase